Sha256: 2cafa7bdc2484bb4ed5604cd747a5eff222ddeb2aca3ac295f376e4501962c32
Contents?: true
Size: 1.29 KB
Versions: 11
Compression:
Stored size: 1.29 KB
Contents
# ====================================== # General parameters to extract Amplicons # ====================================== # Help: <br/>This template is used to get Amplicons<br/> # Help: <br/><b>Plugin list and aplication order:</b><br/> # Help: <ul> # Help: <li>PluginLowHighSize: rejecting too short or too long sequences</li> # Help: <li>PluginKey: trimming Roche 454 sequencing keys (typically 4 first nucleotides)</li> # Help: <li>PluginMids: trimming Roche 454 MIDs and keys</li> # Help: <li>PluginIndeterminations: retaining the longest sequence fragment without indeterminations (N)</li> # Help: <li>PluginAbAdapters: trimming the Roche 454 AB adapters</li> # Help: <li>PluginAmplicons: getting sequences contained between two primers. Sequences with less than two primers are rejected. </li> # Help: <li>PluginLowQuality: trimming low quality regions from sequences. </li> # Help: </ul> plugin_list = PluginLowHighSize,PluginKey,PluginMids,PluginIndeterminations,PluginAbAdapters,PluginAmplicons,PluginLowQuality # do not remove cloned sequences remove_clonality=false # sequences containing with diferent keys (barcodes) are saved to separate folders use_independent_folder_for_each_key=true # remove amplicons containing less or equal number of sequences indicated minimal_repetitions_for_amplicons=1
Version data entries
11 entries across 11 versions & 1 rubygems