README.md in bio-polyploid-tools-0.8.3 vs README.md in bio-polyploid-tools-0.8.4

- old
+ new

@@ -126,10 +126,18 @@ ``` ## Release Notes +### 0.8.4 + +* Added script ```tag_stats.rb`` That gets the descriptive statistics for a tag in a bam file for each reference. + +```bash +ruby tag_stats.rb -b HI.3206.006.Index_2.CS_125RNA_14d_Leaf8.sorted.bam -r /Users/ramirezr/Dropbox/JIC/expVIPMetadatas/RefSeq1.0/Genes/annotation/IWGSCv1.0_UTR_ALL.cdnas.fasta --tag 'NH' +``` + ### 0.8.3 * BUGFIX: ```ChromosomeArm.rb``` was fixed to conform the module assumptions for the package. @@ -169,11 +177,9 @@ * polymarker.rb now validates that all the files exist. * BUGFIX: A reference was required even when it was not used to generate contigs. # Notes - -* BUG: If the SNP is in a gap in the alignment to the chromosomes, it is ignored. * BUG: Blocks with NNNs are picked and treated as semi-specific. * BUG: If the name of the reference have space, the ID is not chopped. ">gene_1 (G12A)" shouls be treated as ">gene_1". * TODO: Add a parameter file to configure the alignments. * TODO: Produce primers for products of different sizes. This can probably be done with the primer_3_preferences option, but hasn't been tested.