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OASIS Table
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And here be MathML: EM4 cells, stably transfected with Flag-HA-DAT, Myc-His-DAT,
or both, were reacted with cross-linker and solubilized in Triton X-100
as above. There is no reason to have this formula,
'bold-italic'Q10=
(M2M1)10/T
1-
T2Required: content unknown here. Five microliters of
anti-Myc 9E10 (Santa Cruz Biotechnology) was added to 1.0 ml of Triton
X-100 extracts, and the mixture was incubated for 1 h at 4°C.
Twenty microliters of rec-protein G Sepharose (Zymed) was added, and the
mixture was incubated for 1 h at 4°C, washed three times in 0.5
ml of lysis buffer, and eluted in 45 μl of 2× Laemmli
sample buffer without reducing agent.
And here be MathML: EM4 cells, stably transfected with Flag-HA-DAT, Myc-His-DAT,
or both, were reacted with cross-linker and solubilized in Triton X-100
as above. There is no reason to have this formula,
'bold-italic'Q10=
(M2M1)10/T
1-
T2Required: content unknown here. Five microliters of
anti-Myc 9E10 (Santa Cruz Biotechnology) was added to 1.0 ml of Triton
X-100 extracts, and the mixture was incubated for 1 h at 4°C.
Twenty microliters of rec-protein G Sepharose (Zymed) was added, and the
mixture was incubated for 1 h at 4°C, washed three times in 0.5
ml of lysis buffer, and eluted in 45 μl of 2× Laemmli
sample buffer without reducing agent.