#!/usr/bin/env ruby require 'optparse' require 'bio-logger' require 'bio-velvet' require 'pp' SCRIPT_NAME = File.basename(__FILE__); LOG_NAME = 'finishm' $:.unshift File.join(File.dirname(__FILE__),'..','lib') require 'priner' # Parse command line options into the options hash options = { :logger => 'stderr', :log_level => 'info', } global = OptionParser.new do |opts| opts.banner = " Usage: #{SCRIPT_NAME} [] FinishM is a collection of tasks related to assembly and metagenome assembly. Common commands: roundup\tImprove a genome by connecting scaffolds and gapfilling visualise\tVisualise the DeBruijn graph Other available commands: wander\tTry to connect contigs (experimental) gapfill\tFill assembly gaps (N characters) (experimental) explore\tWhat happens in the graph beyond the end of my contig(s)? (experimental) Commands for PCR finishing: primers\tdesign primers for multi-primer multi-lane PCR experimental setup (experimental) primers_check\ttest a set of primers for incompatibility (experimental) finish\tprocess results from multi-primer multi-lane PCR experimental setup (experimental) Utility modes: sequence\tGiven a defined sequence of nodes, what is the corresponding sequence? count_paths\tCount the number of paths through assembly graph find_orfs\tFind possible open reading frames in assembly graph \n\n" end global.order! operator = nil subcommands = { 'primers' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Primers.new operator.add_options(opts, options) end}, 'primers_check' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Primers::Checker.new operator.add_options(opts, options) end}, 'finish' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Finisher.new operator.add_options(opts, options) end}, 'gapfill' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::GapFiller.new operator.add_options(opts, options) end}, 'wander' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Wanderer.new operator.add_options(opts, options) end}, 'fluff' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Fluff.new operator.add_options(opts, options) end}, 'explore' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Explorer.new operator.add_options(opts, options) end}, 'assemble' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Assembler.new operator.add_options(opts, options) end}, 'visualise' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Visualise.new operator.add_options(opts, options) end}, 'sequence' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::Sequence.new operator.add_options(opts, options) end}, 'roundup' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::RoundUp.new operator.add_options(opts, options) end}, 'count_paths' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::PathCounter.new operator.add_options(opts, options) end}, 'find_orfs' => lambda {OptionParser.new do |opts| operator = Bio::FinishM::ORFsFinder.new operator.add_options(opts, options) end} } subcommand = nil if ARGV[0] and ARGV[0].match(/finishm$/) #if debugging e.g. 'pry finishm wander ..'. But be careful of finishm no arguments subcommand = ARGV.shift subcommand = ARGV.shift else # not debugging subcommand = ARGV.shift end if subcommand.nil? $stderr.puts global exit 1 elsif !subcommands[subcommand] $stderr.puts "Unrecognized subcommand: #{subcommand}" exit 1 else # Add options specific for subcommand opts = subcommands[subcommand].call # Add global logging options opts.separator "\nVerbosity:\n\n" opts.on("-q", "--quiet", "Run quietly, set logging to ERROR level [default INFO]") {options[:log_level] = 'error'} opts.on("--logger filename",String,"Log to file [default #{options[:logger]}]") { |name| options[:logger] = name} opts.on("--trace options",String,"Set log level [default INFO]. e.g. '--trace debug' to set logging level to DEBUG"){|s| options[:log_level] = s} opts.separator "\n" opts.parse! # Setup logging Bio::Log::CLI.logger(options[:logger]); Bio::Log::CLI.trace(options[:log_level]); log = Bio::Log::LoggerPlus.new(LOG_NAME); Bio::Log::CLI.configure(LOG_NAME); log.outputters.each {|o| o.formatter = Log4r::PatternFormatter.new(:pattern => "%5l %c %d: %m", :date_pattern => '%d/%m %T')} Bio::Log::LoggerPlus.new 'bio-velvet'; Bio::Log::CLI.configure 'bio-velvet' log.debug "Running FinishM #{subcommand} with these options: #{PP.pp(options, "").gsub(/\n$/,'')}" error_message = operator.validate_options(options, ARGV) if error_message.nil? or error_message == false operator.run options, ARGV else $stderr.puts $stderr.puts "ERROR parsing options: #{error_message}" $stderr.puts $stderr.puts opts exit 1 end end