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N,81 ;H@)'Is}u !&)'P lqsy{&}'{P%C{Fd bF &E&- &D O,{ m6 hAi'$T$,$@ $  R 9 / $_ R $ 3 $c O$=^Np=; PF1K${O$ h$1U$ u)' ;ÀA* ` / )'bb C)'UUZzNpk wF uQÀA@z S(nÀA^^ ^F o/o$ )+  $:I  )'uu ~<}uppT R9 JF_k  ÀA 7 9hoq]?s^\Z$3XBE)'))'##U?>ꀀh   QC(_C)'nn yhÀAD 2-7g /w4A z  0j)'""n:GSaXaN66 k{ rDpLBNpk wF uQÀA//7 e6G)' gq3p)'=unable to open first character must be a '>', got ''rallocator::allocate(size_t n) 'n' exceeds maximum supported size> Len = ReverselmumreferencebmaxmatchmummumcandFkthreadsnqthreadssuflinkchildskipLsckmersaveloadmax-chunkversionInvalid parameters.4.0.0beta2-k option valid only for -maxmatchinvalid number of threads specifiedskip parameter was decreased to because skip*K > minimum lengthnote that the skip parameter is very high, a value of or would be more appropriatewarning: very large value for kmer-size: index will be very largekmer size was reduced to because the user set value is too large and cannotbe used in the algorithmERROR -r and -b options are mutually exclusiveindex loaded succesfully WARNING: program does not check the soundness of the reference file for given loaded index. Use the same reference file as used for constructing the index WARNING: some options are now taken from loaded index instead of current user-set values these include: sparseness (-k), suffix links (-suflink), child array (-child) and kmer table size (-kmer).unable to load index construct new index...Usage: [options] . . . [query file32]Implemented MUMmer v3 options:-mum compute maximal matches that are unique in both sequences-mumreference compute maximal matches that are unique in the reference- sequence but not necessarily in the query-sequence (default)-mumcand same as -mumreference-maxmatch compute all maximal matches regardless of their uniqueness-l set the minimum length of a match if not set, the default value is 20-b compute forward and reverse complement matches-F force 4 column output format regardless of the number of reference sequence inputs-n match only the characters a, c, g, or t-L print length of query sequence in header of matches-r compute only reverse complement matches-s print first 53 characters of the matching substring-c Report the query position of a reverse complement match relative to the forward strand of the query sequenceAdditional options:-k sampled suffix positions (one by default)-threads number of threads to use for -maxmatch, only valid k > 1 -qthreads number of threads to use for queries -suflink use suffix links (1=yes or 0=no) in the index and during search [auto]-child use child table (1=yes or 0=no) in the index and during search [auto]-skip sparsify the MEM-finding algorithm even more, performing jumps of skip*k [auto (l-10)/k] this is a performance parameter that trade-offs SA traversal with checking of right-maximal MEMs-kmer use kmer table containing sa-intervals (speeds up searching first k characters) in the index and during search [int value, auto]-save (string) save index to file to use again later (string)-load (string) load index from fileExample usage:./mummer -maxmatch -l 20 -b -n -k 3 -threads 3 ref.fa query.faFind all maximal matches on forward and reverse strandsof length 20 or greater, matching only a, c, t, or g.Index every 3rd position in the ref.fa and use 3 threads to find MEMs.Fastest method for one long query sequence../mummer -maxmatch -l 20 -b -n -k 3 -qthreads 3 ref.fa query.faSame as above, but now use a single thread for every query sequence inquery.fa. 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