Drew, Nancy
Baldo, Danny
Grosberg, Anna
Experimental data for manuscript "Metrics for Assessing Cytoskeletal Orientational Correlations and Consistency"
UC Irvine
2015
COOP
tissue engineering
cardiac
cytoskeleton
Drew;Drew, Nancy
Collection
8224215090
Creative Commons Attribution 4.0 International (CC-BY 4.0)
Experimental data used to validate the co-orientational order parameter (COOP). The data is composed of microscopy images of triangular shaped neonatal rat ventricular myocytes and engineered cardiac tissues.
Neonatal rat ventricular myocytes (NRVMs) prepared from two-day old Sprague-Dawley rats. NRVMs were engineered into isotropic tissues and triangular shaped single cells. These samples were fixed and stained with DAPI, phalloidin, and alpha-actinin. Microscopy images were taken of each stain, and MATLAB was used to determine construct orientation. For detailed methods please refer to the publication (Drew et al., PLosCB, 2015)
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